Characterisation of E12/E47 expression in colorectal cancer
نویسندگان
چکیده
Background In the Western world colorectal cancer is one of the leading causes of cancer-related mortality, and the second most common cancer in women and the third in men. Identification of prognostic markers capable of predicting patient response to chemotherapy is essential to determine more personalised and efficient treatment strategies in colorectal cancer, and may help identify novel therapeutic targets. The aim of the study was to characterise the expression of the E2A transcriptional regulator proteins, E12/E47 (also known as TCF-3 or E2A) in colorectal cancer patient tissue samples. Primarily E12/E47 antibody optimisation was required to allow us to subsequently examine E12/E47 protein expression in colorectal cancer tissue and investigate any correlation with patient outcome.
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Distinct roles for E12 and E47 in B cell specification and the sequential rearrangement of immunoglobulin light chain loci
The E2A gene products, E12 and E47, are critical regulators of B cell development. However, it remains elusive whether E12 and E47 have overlapping and/or distinct functions during B lymphopoiesis. We have generated mice deficient for either E12 or E47 and examined their roles in B cell maturation. We show that E47 is essential for developmental progression at the prepro-B cell stage, whereas E...
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